Cryo-EM sample preparation using traditional support grids has certain problems for specific cases.
- Air-water interface absorption
- Badly preferred orientation
- Sample concentration threshold (>1μM)
- Significant background noise
- Difficulty of reconstructing “small” macromolecules
Shuimu introduces a series of performance-driven graphene grid products - GraFutureTM, to overcome the bottleneck.


GraFuture TM GO
Graphene Oxide Support Film TEM Gird

GraFuture TM RGO
Reduced Graphene Oxide Support Film TEM Gird

GraFuture TM SCG
Single-Crystalline Graphene Support Film TEM Grid
GraFutureTM grids could be used with the following advantages:
-Partial protection from the air-water interface
-More balanced particle orientation distribution
-Low sample concentration (≈100 nM)
-Low background noise
-Successfully solved the structure of sub-100 kDa macromolecules at 2.8 Å resolution
-Providing hexagonal lattice signal, enabling assessment of the cryo-EM image quality & motion correction
GO/RGO SPECS

Ultra-Thin
GO/RGO
TYPE

≤ 5 layers
THICKNESS

≥ 70%
COVERAGE
PERFORMANCES




CASE STUDIES
GO
Cell. 2022 Feb 17;185(4):630-640.e10.
Receptor binding and complex structures of human ACE2 to spike RBD from omicron and delta SARS-CoV-2

RGO
Nature. 2022 Jul;607(7918):399-406.
Structural insights into dsRNA processing by Drosophila Dicer-2-Lo- qs-PD


Nature. 2022 Apr;604(7905):377-383.
Structure deformation and curvature sensing of PIEZO1 in lipid mem- branes


Science. 2021 Jan 8;371(6525):eabe8863.
Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2
