Applications
Cryo-EM has become a standard technique for the structural determination of macromolecules, especially for samples not applicable for X-ray crystallography and NMR due to size, crystallization difficulty, stability, complexity, and conformational variability.
High-resolution 3D structures of proteins, protein complexes and even RNA provide deeper understanding of biological processes, mechanisms of action of molecules, and structure-activity relationships, which guides drug discovery to the right path.
3D structure determination and characterization of small molecules with interacting proteins using cryo-EM assists in omtimizing chemical synthesis, understanding the structure-activity relationship and guiding the design of formulation and drug delivery approaches.
Cryo-EM is an effective technique to map the interactions between antibodies and antigens and has many applications in antibody development and biologics discovery.
Structural data can be used for paratopes/epitopes identification, structure-activity relationship determination, cross-specificity identification, monoclonal antibody optimization and IP protection.
High-resolution structures showing the interaction between antibodies and antigens can also guide vaccine development as an alternative to traditional approaches.
By providing comprehensive and high-resolution structural information, cryo-EM supports the development and manufacturing programs of the design of new drug delivery systems by characterizing the shape, size, size distribution and morphology of samples such as nanoparticles, liposomes, Adeno Associated-Virus (AAV), and other viral-like particles.
Structural characterization by cryo-EM has been acknowledged as an accurate quality control approach in many manufacturing scenarios.
Proteolysis targeting chimera (PROTAC) has drawn great attention due to its potential to target "undruggable" proteins by linking two small-molecule binding ligands.
Structural information of the binding mechanism of small molecules and targeted protein or E3 ligase can significantly improve the understanding of PROTAC technique.
Via cryo-EM, visualization of such binding interaction is possible, providing insight into molecular design and search of new binding sites on the targeted protein.
Cryo-EM SPA
Workflow
Workflow


Why do you need
cryo-EM SPA?
cryo-EM SPA?
Maintain the sample in its near-native state
Require only a small amount of samples
Capture flexible conformations
Determine heterogenous protein complex structures
CASES
Structures Solved by Shuimu
Our platform has delivered high-quality cryo-EM and microED structures to over 200 clients since 2017.
Advanced
Cryo-EM Facility
Cryo-EM Facility
The highest specs microscopes & advanced computing platform for high-quality structure determination
Cutting Edge Scientist Team
A team of PhD scientists from the top institutions, expertised in structural biology, protein science and computation
Profound Experience
200+ cryo-EM projects experience, from membrane proteins to antigen-antibody complexes
Resolution Pursuit
150+ protein structures determined at resolution better than 3.5Å: the best at 1.8Å and the smallest being 51kDA
Shuimu Cryo-EM SPA Advantages
Shuimu Technology
We've developed unique tookits to excel in cryo-EM structure determination.
GraFutureTM
To overcome the bottleneck in cryo-EM sample preparation, we developed a new type of reduced graphene oxide grid to achieve better reproducibility and efficiency of specimen preparation.
modelSMART
We are developing a new differentiable neural network-based method to directly identify the 3D atomic model from a cryo-EM density map in a few hours without any human interference.